Detection of Citrus Exocortis Viroid by Polyacrylamide Gel Electrophoresis
نویسندگان
چکیده
منابع مشابه
Host-directed processing of Citrus exocortis viroid.
Prolonged infection of tomato hybrid (Lycopersicon esculentum x Lycopersicon peruvianum) by Citrus exocortis viroid (CEVd) resulted in viroid-like enlarged structures, detected by gel electrophoresis. This population included two new enlarged variants or D-variants, D-87 and D-76, and three transient species or D-forms, D-38, D-40 and D-43. Sequence analyses exposed a locus near the terminal re...
متن کاملNovel variants of citrus exocortis viroid from southern of Iran
Background and Aims: Citrus exocortis disease is naturally limited to citrus plants and causes a serious disease on sensitive rootstocks such as trifoliate orange. The aim of this study was to sample new variants of citrus exocortis viroid from Fars trees with yellowing and suberization symptoms and comparison with other isolates of this viroid. Materials and Methods: A number of symptomat...
متن کاملogenicity, viroid evolution, Citrus exocortis viroid, Potato spindle tuber viroid, Peach latent mosaic viroid, Chrysan- themum chlorotic mottle viroid
Viroids are autonomously replicating, small singlestranded circular RNA molecules that do not code for proteins and may cause disease in infected, susceptible plants. Viroids have the ability to induce both RNA-mediated transcriptional gene silencing (TGS) and posttranscriptional gene silencing (PTGS) in infected plants. Viroid PTGS has also been demonstrated in a wheat germ extract system. A p...
متن کاملPolyacrylamide Gel Electrophoresis
Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylenebis-acrylamide, commonly called BIS. This process is a freeradical polymerization that requires an initiator, usually ammonium persulfate, and a catalyst like N,N,N’,N’-tetraethylmethylenediamine (TEMED). A distinct advantage of polyacrylamide gel systems is that by ...
متن کاملNeutral polyacrylamide gel electrophoresis.
MATERIALS 5x TBE electrophoresis buffer Polyacrylamide gels are poured and run in 0.5x or 1x TBE at low voltage (1-8 V/cm) to prevent denaturation of small fragments of DNA by Joulic heating. Other electrophoresis buffers such as 1x TAE (please see Agarose Gel Electrophoresis) can be used, but they are not as good as TBE. The gel must be run more slowly in 1x TAE, which does not provide as much...
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ژورنال
عنوان ژورنال: International Organization of Citrus Virologists Conference Proceedings (1957-2010)
سال: 1984
ISSN: 0074-7203,2313-5123
DOI: 10.5070/c56dd2f61k